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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Granzyme H Polyclonal antibody (17228-1-AP)

    WB analysis using 17228-1-AP

    NK-92 cell lysates were subjected to SDS PAGE followed by western blot with 17228-1-AP (GZMH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Granzyme H Polyclonal antibody

Granzyme H Polyclonal Antibody for WB, ELISA
Cat No. 17228-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cathepsin G like 2, CCP X, CGL 2, CGL2, CSP C, CTLA1, CTSGL2, Cytotoxic serine protease C, Granzyme H, GZMH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inNK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17228-1-AP targets Granzyme H in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Granzyme H fusion protein Ag11069 相同性解析による交差性が予測される生物種
Full Name granzyme H (cathepsin G-like 2, protein h-CCPX)
Calculated molecular weight 246 aa, 27 kDa
Observed molecular weight~30 kDa
GenBank accession numberBC027974
Gene Symbol Granzyme H
Gene ID (NCBI) 2999
RRIDAB_3085518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20718
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Granzyme H (GzmH) belongs to a family of 5 human serine proteases that are expressed by cytotoxic immune effector cells. It's regarded as an orphan granzyme with unknown biologic functions in immune defense cells. It's reported that this serine protease is predominantly expressed at high levels in natural killer (NK) cells and has chymotrypsin-like (chymase) activity. The calculated molecular weight is 27 kDa, 17228-1-AP can detect a band around 30 kDa. (PMID: 17409270)

Protocols

Product Specific Protocols
WB protocol for Granzyme H antibody 17228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols