Granzyme K Polyclonal antibody
Granzyme K Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 27894-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | NK-92 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IF | See 1 publications below |
Product Information
27894-1-AP targets Granzyme K in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Granzyme K fusion protein Ag27281 相同性解析による交差性が予測される生物種 |
Full Name | granzyme K (granzyme 3; NK tryptase II) |
Calculated molecular weight | 29 kDa |
GenBank accession number | BC035802 |
Gene symbol | GZMK |
Gene ID (NCBI) | 3003 |
RRID | AB_2881008 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for Granzyme K antibody 27894-1-AP | Download protocol |
IF protocol for Granzyme K antibody 27894-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
iScience CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response |