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  • Featured Product
  • KD/KO Validated

Galectin-1 Polyclonal antibody, PBS Only

Galectin-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11858-1-PBS

Synonyms

GAL1, Gal 1, Beta-galactoside-binding lectin L-14-I, 14 kDa lectin, 14 kDa laminin-binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Galectin-1 Polyclonal antibody (11858-1-AP)

    WB analysis of HepG2 using 11858-1-AP (same clone as 11858-1-PBS)

    WB result of Galectin-1 antibody (11858-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells. This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Galectin-1 Polyclonal antibody (11858-1-AP)

    WB analysis using 11858-1-AP (same clone as 11858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Galectin-1 Polyclonal antibody (11858-1-AP)

    WB analysis using 11858-1-AP (same clone as 11858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of human colon cancer using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of human colon using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of mouse colon using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of mouse colon using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of human colon cancer using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of human lung cancer using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Galectin-1 Polyclonal antibody (11858-1-AP)

    IHC staining of human lung cancer using 11858-1-AP (same clone as 11858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Galectin-1 Polyclonal antibody (11858-1-AP)

    IF Staining of A549 using 11858-1-AP (same clone as 11858-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Galectin-1 antibody (11858-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 11858-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11858-1-PBS targets Galectin-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-1 fusion protein Ag2432 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 1
Calculated molecular weight 15 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC020675
Gene symbol Galectin-1
Gene ID (NCBI) 3956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).