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  • Featured Product
  • KD/KO Validated

Galectin-3 Monoclonal antibody, PBS Only

Galectin-3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 60207-1-PBS

Synonyms

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 35 kDa lectin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin-3 Monoclonal antibody (60207-1-Ig)

    WB analysis using 60207-1-Ig (same clone as 60207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Galectin-3 Monoclonal antibody (60207-1-Ig)

    WB analysis of HeLa cells using 60207-1-Ig (same clone as 60207-1-PBS)

    WB result of Galectin-3 specific antibody (60207-1-Ig, 1:1000) with si-control and si-Galectin-3 transfected HeLa cells. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using Galectin-3 Monoclonal antibody (60207-1-Ig)

    WB analysis of HSC-T6 using 60207-1-Ig (same clone as 60207-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human colon using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human colon using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human ovary tumor using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human ovary tumor using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of mouse kidney using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of mouse kidney using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of mouse kidney using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human thyroid cancer tissue using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of HeLa using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Galectin-3 Monoclonal antibody (60207-1-Ig)

    IF Staining of MCF-7 using 60207-1-Ig (same clone as 60207-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Galectin-3 Monoclonal antibody (60207-1-Ig)

    FC experiment of HeLa using 60207-1-Ig (same clone as 60207-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human Galectin-3 (60207-1-Ig, Clone:1C1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60207-1-PBS targets Galectin-3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Galectin-3 fusion protein Ag6753 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene symbol Galectin-3
Gene ID (NCBI) 3958
RRIDAB_10951109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).