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  • Featured Product
  • KD/KO Validated

Galectin-3 Recombinant antibody, PBS Only

Galectin-3 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4M10

Cat no : 82024-1-PBS

Synonyms

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 4M10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin-3 Recombinant antibody (82024-1-RR)

    WB analysis using 82024-1-RR (same clone as 82024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82024-1-RR (Galectin 3 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Galectin-3 Recombinant antibody (82024-1-RR)

    WB analysis of HeLa using 82024-1-RR (same clone as 82024-1-PBS)

    WB result of Galectin 3 antibody (82024-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using Galectin-3 Recombinant antibody (82024-1-RR)

    IP experiment of MCF-7 using 82024-1-RR (same clone as 82024-1-PBS)

    IP result of anti-Galectin 3 (IP:82024-1-RR, 4ug; Detection:82024-1-RR 1:10000) with MCF-7 cells lysate 1880 ug. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using Galectin-3 Recombinant antibody (82024-1-RR)

    IHC staining of human thyroid cancer using 82024-1-RR (same clone as 82024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82024-1-RR (Galectin 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Galectin-3 Recombinant antibody (82024-1-RR)

    IF Staining of HeLa using 82024-1-RR (same clone as 82024-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin 3 antibody (82024-1-RR, Clone: 4M10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82024-1-RR

    Affinity and Kinetic Characterization of 82024-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82024-1-RR against Human Galectin 3 were performed. The affinity constant is 1.17 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82024-1-PBS targets Galectin-3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Galectin-3 fusion protein Ag6891 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 3
Calculated molecular weight 26 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC001120
Gene symbol Galectin-3
Gene ID (NCBI) 3958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).