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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Galectin-4 Polyclonal antibody (13391-1-AP)

    WB analysis using 13391-1-AP (same clone as 13391-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using Galectin-4 Polyclonal antibody (13391-1-AP)

    WB analysis of COLO 320 using 13391-1-AP (same clone as 13391-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Galectin-4 Polyclonal antibody (13391-1-AP)

    IHC staining of human breast cancer using 13391-1-AP (same clone as 13391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Galectin-4 Polyclonal antibody (13391-1-AP)

    IHC staining of human breast cancer using 13391-1-AP (same clone as 13391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of COLO 320 cells using Galectin-4 Polyclonal antibody (13391-1-AP)

    IF Staining of COLO 320 using 13391-1-AP (same clone as 13391-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using Galectin-4 antibody (13391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13391-1-PBS targets Galectin-4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-4 fusion protein Ag4049 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 4
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC034750
Gene Symbol Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2137072
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56470
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.