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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Galectin-7 Polyclonal antibody

Galectin-7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16730-1-AP

Synonyms

Gal 7, GAL7, Galectin 7, Galectin-7, HKL 14, LGALS7, LGALS7B, p53 induced gene 1 protein, PI7, PIG1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    WB analysis of mouse skin using 16730-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    WB analysis of mouse small intestine using 16730-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervix tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of human cervix using 16730-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using Galectin-7 Polyclonal antibody (16730-1-AP)

    IHC staining of human cervix using 16730-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse skin tissue, mouse small intestine tissue
Positive IHC detected inhuman cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16730-1-AP targets Galectin-7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-7 fusion protein Ag10188 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 7B
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 14-15 kDa
GenBank accession numberBC073743
Gene symbol Galectin-7
Gene ID (NCBI) 653499
RRIDAB_10596781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-7 contains one carbohydrate recognition domain (CRD) and is biologically active as monomer. It is mainly expressed in stratified epithelium, especially in epidermis. Alterations of galectin-7 expression during cancer progression have been reported. Galectin-7 is involved in epithelial differentiation and development and may have a role in tumour development (PMID: 16429325).

Protocols

Product Specific Protocols
WB protocol for Galectin-7 antibody 16730-1-APDownload protocol
IHC protocol for Galectin-7 antibody 16730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols