Galectin-7 Polyclonal antibody
Galectin-7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16730-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skin tissue, mouse small intestine tissue |
Positive IHC detected in | human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
16730-1-AP targets Galectin-7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Galectin-7 fusion protein Ag10188 相同性解析による交差性が予測される生物種 |
Full Name | lectin, galactoside-binding, soluble, 7B |
Calculated molecular weight | 136 aa, 15 kDa |
Observed molecular weight | 14-15 kDa |
GenBank accession number | BC073743 |
Gene symbol | Galectin-7 |
Gene ID (NCBI) | 653499 |
RRID | AB_10596781 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-7 contains one carbohydrate recognition domain (CRD) and is biologically active as monomer. It is mainly expressed in stratified epithelium, especially in epidermis. Alterations of galectin-7 expression during cancer progression have been reported. Galectin-7 is involved in epithelial differentiation and development and may have a role in tumour development (PMID: 16429325).
Protocols
Product Specific Protocols | |
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WB protocol for Galectin-7 antibody 16730-1-AP | Download protocol |
IHC protocol for Galectin-7 antibody 16730-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |