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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Gamma Catenin Polyclonal antibody, PBS Only

Gamma Catenin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11146-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    WB analysis of mouse heart using 11146-1-AP (same clone as 11146-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Gamma Catenin Polyclonal antibody (11146-1-AP)

    WB analysis of A431 using 11146-1-AP (same clone as 11146-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    WB analysis of human heart using 11146-1-AP (same clone as 11146-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using Gamma Catenin Polyclonal antibody (11146-1-AP)

    WB analysis of T-47D using 11146-1-AP (same clone as 11146-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Gamma Catenin Polyclonal antibody (11146-1-AP)

    WB analysis of HeLa using 11146-1-AP (same clone as 11146-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HT-29 cells using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IP experiment of HT-29 using 11146-1-AP (same clone as 11146-1-PBS)

    IP result of anti-Gamma Catenin (IP:11146-1-AP, 4ug; Detection:11146-1-AP 1:1000) with HT-29 cells lysate 1040 ug. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human pancreas cancer using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bowen disease using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Gamma Catenin Polyclonal antibody (11146-1-AP)

    IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11146-1-PBS targets Gamma Catenin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Catenin fusion protein Ag1627 相同性解析による交差性が予測される生物種
Full Name junction plakoglobin
Calculated molecular weight 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC011865
Gene symbol Gamma Catenin
Gene ID (NCBI) 3728
RRIDAB_2265007
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.