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Validation Data Gallery

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  • Western Blot (WB) analysis of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    WB analysis of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    WB analysis of rat stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    rat stomach tissue were subjected to SDS PAGE followed by western blot with 60130-1-Ig (GKN1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IHC staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IHC staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60130-1-Ig (GKN1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of human stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of mouse stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse stomach tissue using Gastrokine 1 Monoclonal antibody (60130-1-Ig)

    IF Staining of mouse stomach using 60130-1-Ig (same clone as 60130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using Gastrokine 1 antibody (60130-1-Ig, Clone: 3C10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60130-1-PBS in a different storage buffer formulation.

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Gastrokine 1 Monoclonal antibody, PBS Only

Gastrokine 1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60130-1-PBS
Clone No.3C10E3

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60130-1-PBS targets Gastrokine 1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gastrokine 1 fusion protein Ag6091 相同性解析による交差性が予測される生物種
Full Name gastrokine 1
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC059778
Gene Symbol Gastrokine 1
Gene ID (NCBI) 56287
RRIDAB_2111459
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS71
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.