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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glucagon Monoclonal antibody, PBS Only

Glucagon Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A8G1

Cat no : 67286-1-PBS

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IHC staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000(under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IHC staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IHC staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67286-1-Ig (Glucagon antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IF Staining of human pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 67286-1-Ig (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 15848-1-AP (Insulin antibody). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IF Staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), INS antibody (15848-1-AP, red). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Glucagon Monoclonal antibody (67286-1-Ig)

    IF Staining of mouse pancreas using 67286-1-Ig (same clone as 67286-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Glucagon antibody (67286-1-Ig, Clone: 2A8G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67286-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67286-1-PBS targets Glucagon in IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glucagon fusion protein Ag10629 相同性解析による交差性が予測される生物種
Full Name glucagon
Calculated molecular weight 180 aa, 21 kDa
GenBank accession numberBC005278
Gene symbol GCG
Gene ID (NCBI) 2641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon from the pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.