Glucocorticoid receptor Monoclonal antibody, PBS Only

Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

4T1 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

HeLa cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

HepG2 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.