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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IHC staining of human ovary cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IHC staining of human ovary cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Glucocorticoid receptor Recombinant antibody (82619-4-RR)

    IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82619-4-RR

    Affinity and Kinetic Characterization of 82619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82619-4-RR against Human Glucocorticoid receptor were performed. The affinity constant is 0.693 nM.

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Glucocorticoid receptor Recombinant antibody, PBS Only

Glucocorticoid receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82619-4-PBS
Clone No.2F22

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

NR3C1, GRL, GR, GCR, GCCR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82619-4-PBS targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight 86 kDa
Observed molecular weight 94-97 kDa
GenBank accession numberBC015610
Gene Symbol Glucocorticoid receptor
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04150
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).