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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of pig liver using 66323-1-Ig (same clone as 66323-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of pig brain using 66323-1-Ig (same clone as 66323-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IF Staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of zebrafish retina using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

    Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1, Glutamine synthetase Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66323-2-PBS. Detection antibody: 66323-1-PBS. Standard:Ag6309. Range: 3.125-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Monoclonal antibody, PBS Only (Detector)

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, Cytometric bead array, Indirect ELISA
Cat No. 66323-1-PBS
Clone No.1D10G8

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, zebrafish

Applications

WB, IHC, IF, Cytometric bead array, Indirect ELISA

Synonyms

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66323-1-PBS targets Glutamine Synthetase as part of a matched antibody pair:

MP50483-1: 66323-2-PBS capture and 66323-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, pig, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881703
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).