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  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Monoclonal antibody, PBS Only (Capture)

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6G4

Cat no : 66323-2-PBS

Synonyms

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    WB analysis of Jurkat using 66323-2-Ig (same clone as 66323-2-PBS)

    WB result of Glutamine synthetase antibody (66323-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of human liver cancer using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of rat liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of rat liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IHC staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IF Staining of mouse liver using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    IF Staining of mouse brain using 66323-2-Ig (same clone as 66323-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Glutamine Synthetase Monoclonal antibody (66323-2-Ig)

    FC experiment of HepG2 using 66323-2-Ig (same clone as 66323-2-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Glutamine Synthetase (66323-2-Ig, Clone:1B6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66323-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1

    Cytometric bead array standard curve of MP50483-1, Glutamine synthetase Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66323-2-PBS. Detection antibody: 66323-1-PBS. Standard:Ag6309. Range: 3.125-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66323-2-PBS targets Glutamine Synthetase as part of a matched antibody pair:

MP50483-1: 66323-2-PBS capture and 66323-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight42 kDa
GenBank accession numberBC011700
Gene symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.