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CoraLite®555-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL555-66323

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite®555-conjugated Glutamine Synthetase Monoc (CL555-66323)

    IF Staining of mouse brain using CL555-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®555 Glutamine Synthetase antibody (CL555-66323, Clone: 1B6G4 ) at dilution of 1:200.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66323 targets Glutamine Synthetase in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
Observed molecular weight42 kDa
GenBank accession numberBC011700
Gene symbol Glutamine Synthetase
Gene ID (NCBI) 2752
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS, GLNS, which belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for the proliferation of fetal skin fibroblasts(PMID:18662667). Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

Protocols

Product Specific Protocols
IF protocol for CL555 Glutamine Synthetase antibody CL555-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols