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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Glutaredoxin 5 Polyclonal antibody (18884-1-AP)

    WB analysis using 18884-1-AP (same clone as 18884-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18884-1-AP (Glutaredoxin 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18884-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Glutaredoxin 5 Polyclonal antibody (18884-1-AP)

    IF Staining of MCF-7 using 18884-1-AP (same clone as 18884-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Glutaredoxin 5 antibody (18884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18884-1-PBS in a different storage buffer formulation.

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Glutaredoxin 5 Polyclonal antibody, PBS Only

Glutaredoxin 5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 18884-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18884-1-PBS targets Glutaredoxin 5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glutaredoxin 5 fusion protein Ag13445 相同性解析による交差性が予測される生物種
Full Name glutaredoxin 5
Calculated molecular weight 157 aa, 17 kDa
Observed molecular weight10-15 kDa
GenBank accession numberBC023528
Gene Symbol GLRX5
Gene ID (NCBI) 51218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SX6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation.