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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GlyT2 Polyclonal antibody

GlyT2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25948-1-AP

Synonyms

SLC6A5, Sodium- and chloride-dependent glycine transporter 2, NET1, GlyT-2, GlyT 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GlyT2 Polyclonal antibody (25948-1-AP)

    WB analysis using 25948-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25948-1-AP (GlyT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GlyT2 Polyclonal antibody (25948-1-AP)

    IHC staining of human skin cancer using 25948-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25948-1-AP (GlyT2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GlyT2 Polyclonal antibody (25948-1-AP)

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GlyT2 Polyclonal antibody (25948-1-AP)

    IF Staining of mouse brain using 25948-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GlyT2 antibody (25948-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25948-1-AP targets GlyT2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GlyT2 fusion protein Ag22835 相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Calculated molecular weight 797 aa, 87 kDa
Observed molecular weight70 kDa
GenBank accession numberBC096319
Gene symbol GlyT2
Gene ID (NCBI) 9152
RRIDAB_3085831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GlyT2 is a transporter protein of the SLC6 family of sodium- and chloride-dependent neurotransmitter transporters. Like other members of the family, GlyT2 is trafficked to the cell surface as homo-oligomers from the endoplasmic reticulum (ER) (PMID: 32714574). GlyT2 is expressed in medulla, and to a lesser extent in spinal cord and cerebellum. GlyT2 has 3 isoforms with the molecular mass of 63, 86 and 87 kDa. The 63 kDa band is the unglycosylated GlyT2 polypeptide, whereas the 70-110 kDa band is a differential glycosylation form of GlyT2 (PMID: 17851090, 17962467).

Protocols

Product Specific Protocols
WB protocol for GlyT2 antibody 25948-1-APDownload protocol
IHC protocol for GlyT2 antibody 25948-1-APDownload protocol
IF protocol for GlyT2 antibody 25948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols