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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glypican 2 Recombinant antibody

Glypican 2 Recombinant Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

230518A4

Cat no : 83241-1-RR

Synonyms

GPC2, Secreted glypican-2, FLJ38962, 230518A4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using Glypican 2 Recombinant antibody (83241-1-RR)

    FC experiment of HeLa using 83241-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Glypican 2 Recombinant antibody (83241-1-RR)

    FC experiment of SH-SY5Y using 83241-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83241-1-RR

    Affinity and Kinetic Characterization of 83241-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive FC (Intra) detected inSH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83241-1-RR targets Glypican 2 in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=1.07 x 105M
Immunogen Glypican 2 fusion protein Ag4041 相同性解析による交差性が予測される生物種
Full Name glypican 2
Calculated molecular weight 579 aa, 63 kDa
Observed molecular weight62，70-100 kDa
GenBank accession numberBC027972
Gene symbol GPC2
Gene ID (NCBI) 221914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

Protocols

Product Specific Protocols
FC protocol for Glypican 2 antibody 83241-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols