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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glypican 3 Polyclonal antibody, PBS Only

Glypican 3 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25175-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HepG2 using 25175-1-AP (same clone as 25175-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Glypican 3 Polyclonal antibody (25175-1-AP)

    WB analysis of HEK-293 using 25175-1-AP (same clone as 25175-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25175-1-AP (Glypican 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25175-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25175-1-PBS targets Glypican 3 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glypican 3 fusion protein Ag10129 相同性解析による交差性が予測される生物種
Full Name glypican 3
Calculated molecular weight 580 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC035972
Gene symbol GPC3
Gene ID (NCBI) 2719
RRIDAB_2879942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, glycanated forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).