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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Glypican-5 Polyclonal antibody (30999-1-AP)

    WB analysis using 30999-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30999-1-AP (GPC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Glypican-5 Polyclonal antibody

Glypican-5 Polyclonal Antibody for WB, ELISA
Cat No. 30999-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GPC5, Secreted glypican-5, glypican 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, NCI-H1299 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30999-1-AP targets Glypican-5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glypican-5 fusion protein Ag34039 相同性解析による交差性が予測される生物種
Full Name glypican 5
Calculated molecular weight 572 aa, 64 kDa
Observed molecular weight63 kDa
GenBank accession numberBC039730
Gene Symbol Glypican 5
Gene ID (NCBI) 2262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP78333
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glypican-5 (GPC5), which is predicted to be located in the cell membrane. In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver (PMID: 9070915). Glypican-5 is a member of heparan sulfate proteoglycans. The repressive role of GPC5 intumorigenesis has been reported in a variety of cancers, including rhabdomyosarcoma, breast cancer, and prostate cancer. GPC5, which is lowly expressed in lung cancer tissues compared with adjacent noncancerous tissues, exhibits a suppressive effect on migration and invasion, and it can also induce G1/S phase arrest of the lung cancer cells in vitro, which is associated with a better prognosis (PMID: 34079082). The molecular weight of Glypican-5 is 63 kDa.

Protocols

Product Specific Protocols
WB protocol for Glypican-5 antibody 30999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols