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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse GM-CSF Recombinant antibody, PBS Only (Capture)

GM-CSF Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230286G9

Cat no : 82861-10-PBS

Synonyms

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00306-3

    Sandwich ELISA standard curve of MP00306-3

    Sandwich ELISA standard curve of MP00306-3, GM-CSF Recombinant Matched Antibody Pair - PBS only. 82861-10-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0512.82861-8-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 pg/mL.

  • Sample test of MP00306-3

    Sample test of MP00306-3

    Spleen and lung from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine. The mean GM-CSF concentration was determined to be 9.5 pg/mL in spleen supernatant, 500.5 pg/mL in lung supernatant.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82861-10-PBS targets GM-CSF as part of a matched antibody pair:

MP00306-3: 82861-10-PBS capture and 82861-8-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name colony stimulating factor 2 (granulocyte-macrophage)
GenBank accession numberNM-009969
Gene symbol GM-CSF
Gene ID (NCBI) 12981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.