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Validation Data Gallery

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  • Western Blot (WB) analysis of NK-92 cells using Granzyme A Recombinant antibody (84583-4-RR)

    WB analysis of NK-92 using 84583-4-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84583-4-RR (GZMA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Granzyme A Recombinant antibody

Granzyme A Recombinant Antibody for WB, ELISA
Cat No. 84583-4-RR
Clone No.241979E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GrA, GZMA, Fragmentin 1, EC:3.4.21.78, Cytotoxic T-lymphocyte proteinase 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84583-4-RR targets Granzyme A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Granzyme A fusion protein Ag1818 相同性解析による交差性が予測される生物種
Full Name granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
Calculated molecular weight 29 kDa
Observed molecular weight27-30 kDa
GenBank accession numberBC015739
Gene Symbol Granzyme A
Gene ID (NCBI) 3001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.

Protocols

Product Specific Protocols
WB protocol for Granzyme A antibody 84583-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols