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Validation Data Gallery

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  • Western Blot (WB) analysis of NK-92 cells using Granzyme B Recombinant antibody (83763-5-RR)

    WB analysis of NK-92 using 83763-5-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 83763-5-RR (GZMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83763-5-RR

    Affinity and Kinetic Characterization of 83763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83763-5-RR against Human Granzyme B were performed. The affinity constant is 0.281 nM.

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Granzyme B Recombinant antibody

Granzyme B Recombinant Antibody for WB, ELISA
Cat No. 83763-5-RR
Clone No.240761C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNK-92 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83763-5-RR targets Granzyme B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC030195
Gene Symbol Granzyme B
Gene ID (NCBI) 3002
RRIDAB_3671358
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

Protocols

Product Specific Protocols
WB protocol for Granzyme B antibody 83763-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols