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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human Granzyme B Rabbit Recombinant Antibody

Granzyme B Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240761G4

Cat no : 98070-1-RR

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR)

    FC experiment of human PBMCs using 98070-1-RR

    1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR)

    FC experiment of human PBMCs using 98070-1-RR

    1x10^6 human PBMCs were stained with APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8) and 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR)

    FC experiment of human PBMCs using 98070-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) (red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98070-1-RR

    Affinity and Kinetic Characterization of 98070-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

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Tested Applications

Positive FC (Intra) detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98070-1-RR is Granzyme B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Calculated molecular weight 247 aa, 28 kDa
GenBank accession numberBC030195
Gene symbol Granzyme B
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

Protocols

Product Specific Protocols
FC protocol for Granzyme B antibody 98070-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols