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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Granzyme K Monoclonal antibody, PBS Only

Granzyme K Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A4

Cat no : 67272-1-PBS

Synonyms

Fragmentin 3, Granzyme 3, Granzyme K, GZMK, NK Tryp 2, NK tryptase 2, TRYP2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IF Staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Granzyme K antibody (67272-1-Ig, Clone: 1C3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67272-1-PBS targets Granzyme K in IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Granzyme K fusion protein Ag27297 相同性解析による交差性が予測される生物種
Full Name granzyme K (granzyme 3; NK tryptase II)
Calculated molecular weight 29 kDa
GenBank accession numberBC035802
Gene symbol GZMK
Gene ID (NCBI) 3003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.