• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Granzyme K Monoclonal antibody, PBS Only

Granzyme K Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C3A4

Cat no : 67272-1-PBS

Synonyms

GZMK, Granzyme3, Granzyme 3, Fragmentin-3, Fragmentin 3

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (Granzyme K antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using Granzyme K Monoclonal antibody (67272-1-Ig)

    IF Staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Granzyme K antibody (67272-1-Ig, Clone: 1C3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67272-1-PBS targets Granzyme K in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Granzyme K fusion protein Ag27297 相同性解析による交差性が予測される生物種
Full Name granzyme K (granzyme 3; NK tryptase II)
Calculated molecular weight 29 kDa
GenBank accession numberBC035802
Gene symbol GZMK
Gene ID (NCBI) 3003
RRIDAB_2882541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.