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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GH1 Polyclonal antibody

GH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27079-1-AP

Synonyms

GH, GH N, GH1, GHN, hGH N, Somatotropin

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    WB analysis of human placenta using 27079-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27079-1-AP (GH1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary adenoma tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human pituitary adenoma using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human placenta using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using GH1 Polyclonal antibody (27079-1-AP)

    IHC staining of human placenta using 27079-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27079-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman pituitary adenoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27079-1-AP is GH1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GH1 fusion protein Ag22841 相同性解析による交差性が予測される生物種
Full Name GH1
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC075012
Gene symbol GH1
Gene ID (NCBI) 2688
RRIDAB_2880745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GH1, also named as GH or GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Protocols

Product Specific Protocols
WB protocol for GH1 antibody 27079-1-APDownload protocol
IHC protocol for GH1 antibody 27079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols