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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMD Polyclonal antibody

GSDMD Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30695-1-AP

Synonyms

M2 4, Gsdmdc1, gasdermin D, Dfna5l, DF5L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Gsdmd Polyclonal antibody (30695-1-AP)

    WB analysis using 30695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Gsdmd Polyclonal antibody (30695-1-AP)

    WB analysis using 30695-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using Gsdmd Polyclonal antibody (30695-1-AP)

    WB analysis of NIH/3T3 using 30695-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of RAW 264.7 cells using Gsdmd Polyclonal antibody (30695-1-AP)

    IP experiment of RAW 264.7 using 30695-1-AP

    IP result of anti-Gsdmd (IP:30695-1-AP, 4ug; Detection:30695-1-AP 1:4000) with RAW 264.7 cells lysate 1400 ug.

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Tested Applications

Positive WB detected inEL-4 cells, EL-4-B5 cells, NIH/3T3 cells, mouse liver tissue
Positive IP detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30695-1-AP targets GSDMD in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSDMD fusion protein Ag33609 相同性解析による交差性が予測される生物種
Full Name gasdermin D
Calculated molecular weight53 kDa
Observed molecular weight50-53 kDa
GenBank accession numberBC029813
Gene symbol Gsdmd
Gene ID (NCBI) 69146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

Protocols

Product Specific Protocols
WB protocol for GSDMD antibody 30695-1-APDownload protocol
IP protocol for GSDMD antibody 30695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols