Histone H1.0 Polyclonal antibody

Name: Histone H1.0 Polyclonal antibody
Brand: Proteintech
SKU: 17510-1-AP

Histone H1.0 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17510-1-AP

Synonyms

H1F0, Histone H1, H1FV, H1-0, H10

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICC

WB analysis of HeLa using 17510-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 17510-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat spleen using 17510-1-AP
rat spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17510-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17510-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17510-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 17510-1-AP
IP result of anti-Histone H1.0 (IP:17510-1-AP, 4ug; Detection:17510-1-AP 1:500) with A431 cells lysate 2400ug.
IHC staining of human colon using 17510-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 17510-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse liver using 17510-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 17510-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 17510-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, Jurkat cells, A431 cells, mouse spleen tissue, rat spleen tissue
Positive IP detected in	A431 cells
Positive IHC detected in	skin cancer **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse liver tissue
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IF	See 1 publications below
ChIP	See 1 publications below

Product Information

17510-1-AP targets Histone H1.0 in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Histone H1.0 fusion protein Ag9982 相同性解析による交差性が予測される生物種
Full Name	H1 histone family, member 0
Calculated molecular weight	21 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC000145
Gene symbol	Histone H1.0
Gene ID (NCBI)	3005
RRID	AB_10695628
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

Protocols

Product Specific Protocols
WB protocol for Histone H1.0 antibody 17510-1-AP	Download protocol
IHC protocol for Histone H1.0 antibody 17510-1-AP	Download protocol
IF protocol for Histone H1.0 antibody 17510-1-AP	Download protocol
IP protocol for Histone H1.0 antibody 17510-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage Authors - Yao Wei View Article
human	WB	Theranostics PMID: 29290796 Authors - Jin Zeng View Article
human	WB	Nucleic Acids Res Pin1 promotes GR transactivation by enhancing recruitment to target genes. Authors - Poolman Toryn M TM View Article
human	WB,IF	Oncogene Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer. Authors - Xianchong Zheng View Article
human	WB	PLoS Pathog Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein. Authors - Linyuan Feng View Article
human	WB	Front Cell Dev Biol GATA4 Regulates Inflammation-Driven Pancreatic Ductal Adenocarcinoma Progression. Authors - Weiliang Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Histone H1.0 Polyclonal antibody

Histone H1.0 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 17510-1-AP

WB analysis of mouse spleen using 17510-1-AP

WB analysis of rat spleen using 17510-1-AP

WB analysis of Jurkat using 17510-1-AP

WB analysis of HeLa using 17510-1-AP

WB analysis of A431 using 17510-1-AP

IP experiment of A431 using 17510-1-AP

IHC staining of human colon using 17510-1-AP

IHC staining of human skin cancer using 17510-1-AP

IF Staining of mouse liver using 17510-1-AP

IF Staining of MCF-7 using 17510-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

PMID: 29290796

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.

Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein.

GATA4 Regulates Inflammation-Driven Pancreatic Ductal Adenocarcinoma Progression.