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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

H2AFY Polyclonal antibody

H2AFY Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26875-1-AP

Synonyms

H2AF12M, H2A/y, H2A.y, H2A histone family, member Y, Core histone macro-H2A.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using H2AFY Polyclonal antibody (26875-1-AP)

    WB analysis using 26875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using H2AFY Polyclonal antibody (26875-1-AP)

    WB analysis of A549 using 26875-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Caco-2 cells using H2AFY Polyclonal antibody (26875-1-AP)

    WB analysis of Caco-2 using 26875-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using H2AFY Polyclonal antibody (26875-1-AP)

    WB analysis of A549 using 26875-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using H2AFY Polyclonal antibody (26875-1-AP)

    WB analysis of mouse brain using 26875-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using H2AFY Polyclonal antibody (26875-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 26875-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26875-1-AP (H2AFY antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using H2AFY Polyclonal antibody (26875-1-AP)

    IF Staining of HepG2 using 26875-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, mouse brain tissue, HeLa cells, rat brain
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26875-1-AP targets H2AFY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen H2AFY fusion protein Ag25445 相同性解析による交差性が予測される生物種
Full Name H2A histone family, member Y
Calculated molecular weight 372 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC013331
Gene symbol H2AFY
Gene ID (NCBI) 9555
RRIDAB_2918113
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for H2AFY antibody 26875-1-APDownload protocol
IHC protocol for H2AFY antibody 26875-1-APDownload protocol
IF protocol for H2AFY antibody 26875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Cell Death Discov

CYB561 promotes HER2+ breast cancer proliferation by inhibiting H2AFY degradation

Authors - Ting Zhao
View Article