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  • Featured Product
  • KD/KO Validated

H6PD Polyclonal antibody

H6PD Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15255-1-AP

Synonyms

6PGL, G6PDH, GDH, H6PD

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using H6PD Polyclonal antibody (15255-1-AP)

    WB analysis of HepG2 using 15255-1-AP

    WB result of H6PD antibody (15255-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-H6PD transfected HepG2 cells.

  • Western Blot (WB) analysis of HepG2 cells using H6PD Polyclonal antibody (15255-1-AP)

    WB analysis of HepG2 using 15255-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using H6PD Polyclonal antibody (15255-1-AP)

    WB analysis of HeLa using 15255-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using H6PD Polyclonal antibody (15255-1-AP)

    WB analysis of mouse liver using 15255-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15255-1-AP (H6PD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using H6PD Polyclonal antibody (15255-1-AP)

    IP experiment of HepG2 using 15255-1-AP

    IP result of anti-H6PD (IP:15255-1-AP, 4ug; Detection:15255-1-AP 1:1000) with HepG2 cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human hepatocirrhosis using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human hepatocirrhosis using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human hepatocirrhosis using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human hepatocirrhosis using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human liver cancer using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using H6PD Polyclonal antibody (15255-1-AP)

    IHC staining of human liver cancer using 15255-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15255-1-AP (H6PD antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15255-1-AP targets H6PD in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen H6PD fusion protein Ag7109 相同性解析による交差性が予測される生物種
Full Name hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)
Calculated molecular weight 89 kDa
Observed molecular weight 89-95 kDa
GenBank accession numberBC081559
Gene symbol H6PD
Gene ID (NCBI) 9563
RRIDAB_10642699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hexose-6-phosphate dehydrogenase (H6PD) is also named as GDH,6PGL. It is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor(PMID:21620971). H6PD is a glycoprotein and has been shown tissue differences in glycosylation status might explain both the difference in migration on SDS-PAGE gels as well as in activity. Defects in H6PD are a cause of cortisone reductase deficiency (CRD). This antibody is specific to H6PD.

Protocols

Product Specific Protocols
WB protocol for H6PD antibody 15255-1-APDownload protocol
IHC protocol for H6PD antibody 15255-1-APDownload protocol
IP protocol for H6PD antibody 15255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
View Article
human,mouseWB

iScience

A fingerprint of 2-[18F]FDG radiometabolites - How tissue-specific metabolism beyond 2-[18F]FDG-6-P could affect tracer accumulation

Authors - Eva-Maria Patronas
  • KD Validated
View Article
WB

Biomolecules

PMID: 31480692

Authors - Xixi Guo
View Article