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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HA Tag Monoclonal antibody, PBS Only

HA Tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C6

Cat no : 66006-2-PBS

Synonyms

YPYDVPDYA

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66006-2-Ig (HA Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.1μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.05μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.025μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ag19172 using HA Tag Monoclonal antibody (66006-2-Ig)

    WB analysis of Ag19172 using 66006-2-Ig (same clone as 66006-2-PBS)

    HA tagged fusion protein was subjected to SDS-PAGE followed by western blot of HA-tag monoclonal antibody 66006-2-Ig at 0.0125μg/mL. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IP experiment of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    IP result of anti-HA Tag (IP:66006-2-Ig, 5ug; Detection:66006-2-Ig 1:2000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Monoclonal antibody (66006-2-Ig)

    IF Staining of Transfected HEK-293 using 66006-2-Ig (same clone as 66006-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (66006-2-Ig, Clone: 1F5C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66006-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66006-2-PBS targets HA Tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name HA Tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.