HA Tag Recombinant antibody

HA Tag Recombinant Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

6I21

Cat no : 81290-1-RR

Synonyms

HA, HA Tag



Tested Applications

Positive WB detected inTransfected HEK-293 cells
Positive IP detected inTransfected HEK-293T cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81290-1-RR targets HA Tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name HA Tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protocols

Product Specific Protocols
WB protocol for HA Tag antibody 81290-1-RRDownload protocol
IF protocol for HA Tag antibody 81290-1-RRDownload protocol
IP protocol for HA Tag antibody 81290-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
humanWB,IF

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng

Free Radic Biol Med

Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury

Authors - Wenyu Shi
humanWB

J Virol

Porcine deltacoronavirus nsp5 antagonizes type I interferon signaling by cleaving IFIT3

Authors - Haixin Huang
WB

Mol Nutr Food Res

Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer

Authors - Da Huang

J Biol Chem

5-methylcytosine transferase NSUN2 drives NRF2-mediated ferroptosis resistance in non-small cell lung cancer

Authors - Youming Chen