ChromoTek HA VHH, recombinant binding protein

Alpaca anti-HA-Tag VHH, purified recombinant binding protein

Specificity

HA-Tag (sequence YPYDVPDYA)

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Cat no : at

Synonyms

Hemagglutinin tag, HA, HA-tag, HA Nanobody



Product Information

Alpaca anti-HA-Tag VHH, purified recombinant binding protein

ApplicationsSuitable for conjugation to beads, plates, and surfaces via NHS-chemistry
Specificity/TargetBinds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions
Molecular Weight14.1 kDa
RRIDAB_3096297
Storage Buffer25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09% sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!