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- KD/KO Validated
HACE1 Polyclonal antibody
HACE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 24104-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
Product Information
The immunogen of 24104-1-AP is HACE1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HACE1 fusion protein Ag21130 相同性解析による交差性が予測される生物種 |
| Full Name | HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 |
| Calculated molecular weight | 909 aa, 102 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC034982 |
| Gene symbol | HACE1 |
| Gene ID (NCBI) | 57531 |
| RRID | AB_2879432 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HACE1(E3 ubiquitin-protein ligase HACE1) also named as KIAA1320, has a tumor-suppressor function dependent on its E3 ligase activity and controlling cell cycle progression during cell stress through degradation of cyclin D1. It belongs to the HECT family of E3 ubiqutin protein ligase. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 102 kDa, 36 kDa, 64 kDa and 78 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HACE1 antibody 24104-1-AP | Download protocol |
| IHC protocol for HACE1 antibody 24104-1-AP | Download protocol |
| IF protocol for HACE1 antibody 24104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Reprod Biol Endocrinol NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes
| ||
Acta Biochim Biophys Sin (Shanghai) Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression | ||
Cancer Med Overexpression of HACE1 in gastric cancer inhibits tumor aggressiveness by impeding cell proliferation and migration.
| ||
Transl Cancer Res Unveiling the role of HACE1 in cervical cancer: implications for human papillomavirus infection and prognosis |
