• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK293 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of Jurkat using 60250-1-Ig (same clone as 60250-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1, HADHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60250-1-PBS. Detection antibody: 60250-2-PBS. Standard:Ag1211. Range: 1.563-100 ng/mL.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

HADHA Monoclonal antibody, PBS Only (Capture)

HADHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 60250-1-PBS
Clone No.3E9B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GBP, ECHA, EC:2.3.1.-, 78 kDa gastrin-binding protein, 78 kDa gastrin binding protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【全製品対象☆】小さなギフトがついてくる！春の特典キャンペーン開催中

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60250-1-PBS targets HADHA as part of a matched antibody pair:

MP50412-1: 60250-1-PBS capture and 60250-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HADHA fusion protein Ag1211 相同性解析による交差性が予測される生物種
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight 83 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC009235
Gene Symbol HADHA
Gene ID (NCBI) 3030
RRIDAB_2881371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40939
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).