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  • Featured Product
  • KD/KO Validated

HADHA Monoclonal antibody, PBS Only (Capture)

HADHA Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B1

Cat no : 60250-1-PBS

Synonyms

GBP, ECHA, EC:2.3.1.-, 78 kDa gastrin-binding protein, 78 kDa gastrin binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of Jurkat using 60250-1-Ig (same clone as 60250-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1

    Cytometric bead array standard curve of MP50412-1, HADHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60250-1-PBS. Detection antibody: 60250-2-PBS. Standard:Ag1211. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60250-1-PBS targets HADHA as part of a matched antibody pair:

MP50412-1: 60250-1-PBS capture and 60250-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HADHA fusion protein Ag1211 相同性解析による交差性が予測される生物種
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight 83 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC009235
Gene symbol HADHA
Gene ID (NCBI) 3030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).