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  • Featured Product
  • KD/KO Validated

HADHA Monoclonal antibody, PBS Only

HADHA Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E9B1

Cat no : 60250-1-PBS

Synonyms

78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK293 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

    WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of A375 using 60250-1-Ig (same clone as 60250-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HADHA Monoclonal antibody (60250-1-Ig)

    WB analysis of Jurkat using 60250-1-Ig (same clone as 60250-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of human heart using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of human liver using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using HADHA Monoclonal antibody (60250-1-Ig)

    IHC staining of human liver using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 60250-1-Ig(HADHA antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HADHA Monoclonal antibody (60250-1-Ig)

    IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60250-1-PBS targets HADHA in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HADHA fusion protein Ag1211 相同性解析による交差性が予測される生物種
Full Name hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Calculated molecular weight 83 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC009235
Gene symbol HADHA
Gene ID (NCBI) 3030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).