HAO1 Polyclonal antibody
HAO1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25056-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, rat liver tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 25056-1-AP is HAO1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HAO1 fusion protein Ag21884 相同性解析による交差性が予測される生物種 |
Full Name | hydroxyacid oxidase (glycolate oxidase) 1 |
Calculated molecular weight | 370 aa, 41 kDa |
Observed molecular weight | 41 kDa |
GenBank accession number | BC113665 |
Gene symbol | HAO1 |
Gene ID (NCBI) | 54363 |
RRID | AB_2879875 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for HAO1 antibody 25056-1-AP | Download protocol |
IHC protocol for HAO1 antibody 25056-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |