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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAO1 Polyclonal antibody

HAO1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25056-1-AP

Synonyms

Glycolate oxidase, GOX, GOX1, HAO1, HAOX1, Hydroxyacid oxidase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using HAO1 Polyclonal antibody (25056-1-AP)

    WB analysis of mouse liver using 25056-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using HAO1 Polyclonal antibody (25056-1-AP)

    WB analysis of rat liver using 25056-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 25056-1-AP (HAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25056-1-AP is HAO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAO1 fusion protein Ag21884 相同性解析による交差性が予測される生物種
Full Name hydroxyacid oxidase (glycolate oxidase) 1
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC113665
Gene symbol HAO1
Gene ID (NCBI) 54363
RRIDAB_2879875
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAO1 antibody 25056-1-APDownload protocol
IHC protocol for HAO1 antibody 25056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols