Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, mouse kidney tissue, HepG2 cells, rat kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
16563-1-AP targets HAO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HAO2 fusion protein Ag9838 相同性解析による交差性が予測される生物種 |
| Full Name | hydroxyacid oxidase 2 (long chain) |
| Calculated molecular weight | 351 aa, 39 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC020863 |
| Gene Symbol | HAO2 |
| Gene ID (NCBI) | 51179 |
| RRID | AB_3669250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HAO2 antibody 16563-1-AP | Download protocol |
| IF protocol for HAO2 antibody 16563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |