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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAPLN1 Polyclonal antibody

HAPLN1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26831-1-AP

Synonyms

Cartilage link protein, Cartilage linking protein 1, CRTL1, HAPLN1, Proteoglycan link protein

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse cerebellum using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse cerebellum using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse placenta using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using HAPLN1 Polyclonal antibody (26831-1-AP)

    IHC staining of mouse placenta using 26831-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HAPLN1 Polyclonal antibody (26831-1-AP)

    IF Staining of U2OS using 26831-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HAPLN1 antibody (26831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inmouse placenta tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26831-1-AP targets HAPLN1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAPLN1 fusion protein Ag25221 相同性解析による交差性が予測される生物種
Full Name hyaluronan and proteoglycan link protein 1
Calculated molecular weight 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC057808
Gene symbol HAPLN1
Gene ID (NCBI) 1404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453).

Protocols

Product Specific Protocols
IHC protocol for HAPLN1 antibody 26831-1-APDownload protocol
IF protocol for HAPLN1 antibody 26831-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols