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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HARBI1 Recombinant antibody (84482-4-RR)

    WB analysis using 84482-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84482-4-RR (HARBI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84482-4-RR

    Affinity and Kinetic Characterization of 84482-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84482-4-RR against Human HARBI1 were performed. The affinity constant is 78.4 pM.

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HARBI1 Recombinant antibody

HARBI1 Recombinant Antibody for WB, ELISA
Cat No. 84482-4-RR
Clone No.241915C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Putative nuclease HARBI1, Harbinger transposase-derived nuclease, EC:3.1.-.-, C11orf77, 241915C10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84482-4-RR targets HARBI1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HARBI1 fusion protein Ag34137 相同性解析による交差性が予測される生物種
Full Name harbinger transposase derived 1
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC036925
Gene Symbol HARBI1
Gene ID (NCBI) 283254
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96MB7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HARBI1, also named as harbinger transposase derived 1, is predicted to enable metal ion binding activity and nuclease activity. HARBI1 is predicted to be involved in nucleic acid phosphodiester bond hydrolysis. [provided by Alliance of Genome Resources, Apr 2022] In plants, HARBI1 genes have developed new cellular functions to benefit the host, certain HARBI1 genes in rice and Arabidopsis thaliana ( A. thaliana) have been reported to play critical roles in regulating plant growth and development.(PMID:37950159)

Protocols

Product Specific Protocols
WB protocol for HARBI1 antibody 84482-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols