HARS Polyclonal antibody

Name: HARS Polyclonal antibody
Brand: Proteintech
SKU: 16375-1-AP

HARS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16375-1-AP

Synonyms

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

Validation Data Gallery

Filter:

AllWBIHC

WB analysis of mouse liver using 16375-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16375-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16375-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16375-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 16375-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16375-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16375-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 16375-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16375-1-AP (HARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

-/ -

Cat No. 16375-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, human kidney tissue, K-562 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

16375-1-AP targets HARS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HARS fusion protein Ag9333 相同性解析による交差性が予測される生物種
Full Name	histidyl-tRNA synthetase
Calculated molecular weight	509 aa, 57 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC011807
Gene symbol	HARS
Gene ID (NCBI)	3035
RRID	AB_2116283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Protocols

Product Specific Protocols
WB protocol for HARS antibody 16375-1-AP	Download protocol
IHC protocol for HARS antibody 16375-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Authors - Maria C Passarelli View Article
mouse	WB	Sci Rep NRF2 activation ameliorates blood-brain barrier injury after cerebral ischemic stroke by regulating ferroptosis and inflammation Authors - Wei Fan View Article
mouse	WB	Neuroscience MyD88 inhibition attenuates cerebral ischemia-reperfusion injury by regulating the inflammatory response and reducing blood-brain barrier damage Authors - Fangchao Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HARS Polyclonal antibody

HARS Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of mouse liver using 16375-1-AP

WB analysis of mouse brain using 16375-1-AP

WB analysis of human kidney using 16375-1-AP

WB analysis of HeLa using 16375-1-AP

WB analysis of mouse heart using 16375-1-AP

WB analysis of mouse lung using 16375-1-AP

WB analysis of K-562 using 16375-1-AP

IHC staining of human stomach using 16375-1-AP