Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse liver using 16375-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16375-1-AP
WB result of HARS antibody (16375-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HARS transfected HeLa cells.
WB analysis of mouse heart using 16375-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16375-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16375-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16375-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16375-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16375-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human stomach using 16375-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16375-1-AP (HARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

HARS Polyclonal antibody

HARS Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16375-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, human kidney tissue, K-562 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

16375-1-AP targets HARS in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	HARS fusion protein Ag9333 相同性解析による交差性が予測される生物種
Full Name	histidyl-tRNA synthetase
Calculated molecular weight	509 aa, 57 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC011807
Gene Symbol	HARS
Gene ID (NCBI)	3035
RRID	AB_2116283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P12081
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. The D364Y (Asp364-to-Tyr) mutation of HARS1 inhibits morphological differentiation of neuronal cells (PMID: 35380399).

Protocols

Product Specific Protocols
WB protocol for HARS antibody 16375-1-AP	Download protocol
IHC protocol for HARS antibody 16375-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. Authors - Maria C Passarelli View Article
mouse	WB	Sci Rep NRF2 activation ameliorates blood-brain barrier injury after cerebral ischemic stroke by regulating ferroptosis and inflammation Authors - Wei Fan View Article
mouse	WB	Neuroscience MyD88 inhibition attenuates cerebral ischemia-reperfusion injury by regulating the inflammatory response and reducing blood-brain barrier damage Authors - Fangchao Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 16375-1-AP

WB analysis of HeLa using 16375-1-AP

WB analysis of mouse heart using 16375-1-AP

WB analysis of mouse lung using 16375-1-AP

WB analysis of K-562 using 16375-1-AP

WB analysis of mouse brain using 16375-1-AP

WB analysis of human kidney using 16375-1-AP