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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HARS Monoclonal antibody

HARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat no : 67662-1-Ig

Synonyms

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HARS Monoclonal antibody (67662-1-Ig)

    WB analysis using 67662-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HARS Monoclonal antibody (67662-1-Ig)

    IF Staining of MCF-7 using 67662-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67662-1-Ig targets HARS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HARS fusion protein Ag9224 相同性解析による交差性が予測される生物種
Full Name histidyl-tRNA synthetase
Calculated molecular weight 509 aa, 57 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC011807
Gene symbol HARS
Gene ID (NCBI) 3035
RRIDAB_2882859
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Protocols

Product Specific Protocols
WB protocol for HARS antibody 67662-1-IgDownload protocol
IF protocol for HARS antibody 67662-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols