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  • Featured Product
  • KD/KO Validated

HARS2 Polyclonal antibody

HARS2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11301-1-AP

Synonyms

HARS2, HARSL, HARSR, HisRS, Histidine tRNA ligase, Histidine tRNA ligase like, HO3

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of MCF-7 using 11301-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of HEK-293 using 11301-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of human heart using 11301-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of MCF-7 using 11301-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of mouse kidney using 11301-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HARS2 Polyclonal antibody (11301-1-AP)

    WB analysis of HEK-293 using 11301-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HARS2 Polyclonal antibody (11301-1-AP)

    IP experiment of HEK-293 using 11301-1-AP

    IP result of anti-HARS2 (IP:11301-1-AP, 4ug; Detection:11301-1-AP 1:500) with HEK-293 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HARS2 Polyclonal antibody (11301-1-AP)

    IHC staining of human breast cancer using 11301-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HARS2 Polyclonal antibody (11301-1-AP)

    IHC staining of human breast cancer using 11301-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HARS2 Polyclonal antibody (11301-1-AP)

    IHC staining of human breast cancer using 11301-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HARS2 Polyclonal antibody (11301-1-AP)

    IHC staining of human kidney using 11301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using HARS2 Polyclonal antibody (11301-1-AP)

    IHC staining of human kidney using 11301-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inMCF7 cells, HEK-293 cells, human heart tissue, MCF-7 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11301-1-AP targets HARS2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HARS2 fusion protein Ag1827 相同性解析による交差性が予測される生物種
Full Name histidyl-tRNA synthetase 2, mitochondrial (putative)
Calculated molecular weight 57 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC014982
Gene symbol HARS2
Gene ID (NCBI) 23438
RRIDAB_10640530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for HARS2 antibody 11301-1-APDownload protocol
IHC protocol for HARS2 antibody 11301-1-APDownload protocol
IP protocol for HARS2 antibody 11301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
View Article
mouseWB

Front Cell Neurosci

Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.

Authors - Pengcheng Xu
  • KO Validated
View Article
mouseWB

Cells

CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2

Authors - Jana Key
View Article