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  • KD/KO Validated

HAT1 Polyclonal antibody

HAT1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 11432-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

KAT1, EC:2.3.1.48

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MDA-MB-231 cells, NIH/3T3 cells, C6 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse ovary tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11432-1-AP targets HAT1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAT1 fusion protein Ag1952 相同性解析による交差性が予測される生物種
Full Name histone acetyltransferase 1
Calculated molecular weight 419 aa, 50 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC018682
Gene Symbol HAT1
Gene ID (NCBI) 8520
RRIDAB_2116435
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAT1 antibody 11432-1-APDownload protocol
IHC protocol for HAT1 antibody 11432-1-APDownload protocol
IF protocol for HAT1 antibody 11432-1-APDownload protocol
IP protocol for HAT1 antibody 11432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
humanWB

EMBO Rep

Histone acetyltransferase 1 is a succinyltransferase for histones and non-histones and promotes tumorigenesis.

Authors - Guang Yang
  • KD Validated
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu
ratIHC

Cereb Cortex

New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.

Authors - Ben Shabat Moti
mouseWB

Alcohol

Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.

Authors - Minjie Chen
humanIF

Biochem Biophys Res Commun

Identification and characterization of an endoplasmic reticulum localization motif.

Authors - Yong-Wei Nie
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