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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAT1 Monoclonal antibody (67971-1-Ig)

    WB analysis using 67971-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67971-1-Ig (HAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using HAT1 Monoclonal antibody (67971-1-Ig)

    WB analysis of HEK-293 using 67971-1-Ig

    WB result of HAT1 antibody (67971-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells.

  • Immunohistochemistry (IHC) staining of rat testis tissue using HAT1 Monoclonal antibody (67971-1-Ig)

    IHC staining of rat testis using 67971-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67971-1-Ig (HAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

HAT1 Monoclonal antibody

HAT1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67971-1-Ig
Clone No.1A11F6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

KAT1, EC:2.3.1.48, 1A11F6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67971-1-Ig targets HAT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HAT1 fusion protein Ag1952 相同性解析による交差性が予測される生物種
Full Name histone acetyltransferase 1
Calculated molecular weight 419 aa, 50 kDa
Observed molecular weight45-46 kDa
GenBank accession numberBC018682
Gene Symbol HAT1
Gene ID (NCBI) 8520
RRIDAB_2918721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAT1 antibody 67971-1-IgDownload protocol
IHC protocol for HAT1 antibody 67971-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols