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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HAUS4 Polyclonal antibody

HAUS4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20104-1-AP

Synonyms

C14orf94, HAUS4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    WB analysis of human brain using 20104-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    WB analysis of mouse lung using 20104-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    WB analysis of human placenta using 20104-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    WB analysis of mouse skin using 20104-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20104-1-AP (HAUS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human prostate cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human placenta using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20104-1-AP (HAUS4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human colon cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human colon cancer using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human placenta using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HAUS4 Polyclonal antibody (20104-1-AP)

    IHC staining of human placenta using 20104-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20104-1-AP (HAUS4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman brain tissue, human placenta tissue, mouse lung tissue, mouse skin tissue
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20104-1-AP is HAUS4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAUS4 fusion protein Ag13717 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 94
Calculated molecular weight 363 aa, 42 kDa
Observed molecular weight 28 kDa, 37 kDa, 42 kDa
GenBank accession numberBC002554
Gene symbol C14orf94
Gene ID (NCBI) 54930
RRIDAB_10639366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HAUS4, also named as C14orf94, has four isoforms with MW 27-29 kDa, 37 kDa and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for HAUS4 antibody 20104-1-APDownload protocol
IHC protocol for HAUS4 antibody 20104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Reconstitution and mechanistic dissection of the human microtubule branching machinery.

Authors - Yaqian Zhang
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article