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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Hemoglobin Alpha-Specific Polyclonal antibody (19990-1-AP)

    WB analysis of human heart using 19990-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Hemoglobin Alpha-Specific Polyclonal antibody (19990-1-AP)

    WB analysis of human placenta using 19990-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using HBA1-Specific Polyclonal antibody (19990-1-AP)

    WB analysis of human spleen using 19990-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 19990-1-AP (HBA1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon using Hemoglobin Alpha-Specific Polyclonal antibody (19990-1-AP)

    IHC staining of human colon using 19990-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 19990-1-AP (Hemoglobin Alpha-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Hemoglobin Alpha-Specific Polyclonal antibody

Hemoglobin Alpha-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19990-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HBA1, Hemoglobin Alpha, Hemoglobin a, HBA2, Alpha-globin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman placenta tissue, human heart tissue, human spleen tissue
Positive IHC detected inhuman colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19990-1-AP targets Hemoglobin Alpha-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name hemoglobin, alpha 1
Calculated molecular weight 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberNM_000558
Gene Symbol HBA1
Gene ID (NCBI) 3039
RRIDAB_11182500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP69905
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). The antibody is specific to HBA1and has no cross reaction to other globin member.

Protocols

Product Specific Protocols
WB protocol for Hemoglobin Alpha-Specific antibody 19990-1-APDownload protocol
IHC protocol for Hemoglobin Alpha-Specific antibody 19990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols