Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue, human placenta, human peripheral blood platelets, human plasma, rabbit brain, rabbit liver |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67899-1-Ig targets HBB in WB, ELISA applications and shows reactivity with Human, rabbit samples.
| Tested Reactivity | Human, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HBB fusion protein Ag9216 相同性解析による交差性が予測される生物種 |
| Full Name | hemoglobin, beta |
| Calculated molecular weight | 147 aa, 16 kDa |
| Observed molecular weight | 14-16 Da |
| GenBank accession number | BC007075 |
| Gene Symbol | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_2918655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P68871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HBB antibody 67899-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |