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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HBD Polyclonal antibody

HBD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16824-1-AP

Synonyms

Delta globin, HBD, Hemoglobin delta chain, Hemoglobin subunit delta, hemoglobin, delta

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human plasma using HBD Polyclonal antibody (16824-1-AP)

    WB analysis of human plasma using 16824-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using HBD Polyclonal antibody (16824-1-AP)

    WB analysis of human plasma using 16824-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16824-1-AP (HBD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using HBD Polyclonal antibody (16824-1-AP)

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using HBD Polyclonal antibody (16824-1-AP)

    IHC staining of human liver using 16824-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16824-1-AP (HBD antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman blood tissue, human plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16824-1-AP targets HBD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HBD fusion protein Ag10409 相同性解析による交差性が予測される生物種
Full Name hemoglobin, delta
Calculated molecular weight 147 aa, 16 kDa
Observed molecular weight 16 kDa
GenBank accession numberBC070282
Gene symbol HBD
Gene ID (NCBI) 3045
RRIDAB_2114476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HBD antibody 16824-1-APDownload protocol
IHC protocol for HBD antibody 16824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Pharmacol

Beneficial Effect of Edoxaban on Preventing Atrial Fibrillation and Coagulation by Reducing Inflammation via HBG1/HBD Biomarkers.

Authors - Chenguang Yang
View Article
humanWB

Placenta

SWATH proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy

Authors - Yuxuan Jiang
View Article