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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBQ1 Polyclonal antibody (16706-1-AP)

    WB analysis using 16706-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16706-1-AP (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HBQ1 Polyclonal antibody (16706-1-AP)

    IHC staining of human placenta using 16706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16706-1-AP (HBQ1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using HBQ1 Polyclonal antibody (16706-1-AP)

    IHC staining of human placenta using 16706-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16706-1-AP (HBQ1 antibody) at dilution of 1:200 (under 40x lens).

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HBQ1 Polyclonal antibody

HBQ1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16706-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Theta 1 globin, hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16706-1-AP targets HBQ1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HBQ1 fusion protein Ag10105 相同性解析による交差性が予測される生物種
Full Name hemoglobin, theta 1
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight10~16 kDa
GenBank accession numberBC056686
Gene Symbol HBQ1
Gene ID (NCBI) 3049
RRIDAB_1851212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

Protocols

Product Specific Protocols
WB protocol for HBQ1 antibody 16706-1-APDownload protocol
IHC protocol for HBQ1 antibody 16706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols