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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HBQ1 Recombinant antibody

HBQ1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240527D6

Cat no : 83576-4-RR

Synonyms

hemoglobin, theta 1, Hemoglobin theta-1 chain, Hemoglobin theta 1 chain, Hemoglobin subunit theta-1, Hemoglobin subunit theta 1

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using HBQ1 Recombinant antibody (83576-4-RR)

    WB analysis of K-562 using 83576-4-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83576-4-RR

    Affinity and Kinetic Characterization of 83576-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

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Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83576-4-RR is HBQ1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HBQ1 fusion protein Ag10105 相同性解析による交差性が予測される生物種
Full Name hemoglobin, theta 1
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight10~16 kDa
GenBank accession numberBC056686
Gene symbol HBQ1
Gene ID (NCBI) 3049
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

Protocols

Product Specific Protocols
WB protocol for HBQ1 antibody 83576-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols